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Test No. 437: Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage
Test No. 437 is a guideline that describes the Bovine Corneal Opacity and Permeability Test (BCOP), which is used to evaluate the potential eye irritancy of chemicals or substances. The guideline provides instructions for using bovine corneas, applying the test substance to the corneas, and assessing changes in corneal opacity and permeability. This test helps identify substances that can cause eye irritation, providing important information for assessing their potential hazards to the eyes and guiding safety measures.
Test No. 467: Defined Approaches for Serious Eye Damage and Eye Irritation
Test No. 467 is a guideline that describes Defined Approaches for assessing Serious Eye Damage and Eye Irritation. Rather than a single test method, it provides a framework for integrating multiple alternative methods, such as in vitro tests, computational models, and existing data, to determine the potential hazard of chemicals or substances to the eyes. This approach helps evaluate the likelihood of serious eye damage or irritation caused by substances, providing a more comprehensive and efficient assessment while reducing the need for animal testing.
Test No. 491: Short Time Exposure In Vitro Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage
Test No. 491 is a guideline that outlines the Short Time Exposure In Vitro Test Method for identifying chemicals that either induce serious eye damage or do not require classification for eye irritation or serious eye damage. This test provides a time-efficient approach using in vitro models, such as 3D tissue constructs or cell monolayers, to evaluate the potential effects of chemicals on the eyes. It helps determine the hazard potential of chemicals without the need for animal testing, aiding in the classification and labeling of substances based on their eye irritation or damage potential.
Test No. 492: Reconstructed human Cornea-like Epithelium (RhCE) test method for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage
This Test Guideline describes an in vitro procedure allowing the identification of chemicals (substances and mixtures) not requiring classification and labelling for eye irritation or serious eye damage in accordance with UN GHS. It makes use of reconstructed human cornea-like epithelium (RhCE) which closely mimics the histological, morphological, biochemical and physiological properties of the human corneal epithelium. The test evaluates the ability of a test chemical to induce cytotoxicity in a RhCE tissue construct, as measured by the MTT assay.
Test No. 492B: Reconstructed Human Cornea-like Epithelium (RHCE) Test Method for Eye Hazard Identification
Test No. 492B is a guideline that describes the Reconstructed Human Cornea-like Epithelium (RHCE) Test Method for identifying potential eye hazards. This test is an in vitro approach using a three-dimensional model of human cornea-like epithelium to assess the eye irritation potential of chemicals or substances. The guideline provides instructions for exposing the RHCE model to the test substance and evaluating various endpoints, such as tissue viability and histopathological changes. The RHCE test helps identify substances that may cause eye irritation, providing valuable information for evaluating their potential hazards to the eyes and guiding safety measures.
Test No. 496: In vitro Macromolecular Test Method for Identifying Chemicals Inducing Serious Eye Damage and Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage
Test No. 496 is a guideline that outlines the In vitro Macromolecular Test Method for identifying chemicals that induce serious eye damage or do not require classification for eye irritation or serious eye damage. This test utilizes macromolecules such as proteins or peptides to simulate the ocular environment and assess the potential effects of chemicals on the eyes. The guideline provides instructions for exposing the macromolecules to the test substance and evaluating various endpoints, such as protein denaturation or aggregation. This test helps determine the hazard potential of chemicals to the eyes without the need for animal testing, aiding in the classification and labeling of substances based on their eye irritation or damage potential.