Endocrine Disruption Assays
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OECD Test No. 455: Performance-Based Test Guideline for Stably Transfected Transactivation In Vitro Assays to Detect Estrogen Receptor Agonists and Antagonists
Test No. 455 is a Performance-Based Test Guideline that outlines a standardized method for conducting in vitro assays to detect estrogen receptor agonists and antagonists in stably transfected cells. This test is crucial for evaluating the potential estrogenic activity of chemicals, such as those found in pharmaceuticals or environmental contaminants, and determining their ability to interact with estrogen receptors in the body.
OECD Test No. 456: H295R Steroidogenesis Assay
Test No. 456 is a guideline that describes the H295R Steroidogenesis Assay, which is used to assess the potential of chemicals to interfere with steroid hormone production in the human adrenal cortex. The guideline provides detailed instructions on the cultivation and treatment of H295R cells, as well as the measurement and analysis of steroid hormone production using various analytical techniques. This assay is valuable for evaluating the endocrine-disrupting effects of substances, such as pharmaceuticals, pesticides, or industrial chemicals, and helps in assessing their impact on human health and the environment.
OECD Test No. 457 : Test No. 457: BG1Luc Estrogen Receptor Transactivation Test Method for Identifying Estrogen Receptor Agonists and Antagonists
Test No. 457 is a guideline that outlines the Yeast Estrogen Screen (YES) assay, which is used to evaluate the estrogenic activity of chemicals or substances. The guideline provides a standardized protocol for conducting the assay using genetically modified yeast cells that express estrogen receptors. The assay involves measuring the growth response of the yeast cells when exposed to test substances, and the results help identify chemicals that can mimic or interfere with estrogenic activity. The YES assay is a rapid and cost-effective screening tool for identifying potential endocrine-disrupting compounds.
OECD Test No: 458: Stably Transfected Human Androgen Receptor Transcriptional Activation Assay for Detection of Androgenic Agonist and Antagonist Activity of Chemicals
Test No. 458 is a guideline that outlines the Androgen Receptor Transactivation assay, which is used to assess the potential of chemicals to interact with the androgen receptor and modulate its activity. The guideline provides instructions for conducting the assay using a suitable cell line that expresses the androgen receptor and a reporter gene. The assay measures the ability of test substances to activate or inhibit the androgen receptor, providing insights into their androgenic or anti-androgenic effects. This assay is crucial for evaluating the endocrine-disrupting potential of chemicals, particularly those that may impact the male reproductive system or have hormonal effects.
Test No. 493, also known as US EPA TG OPPTS 890.1150, is a guideline that provides a standardized method for determining the binding affinity of chemicals to estrogen or androgen receptors. The guideline outlines the experimental procedures, including the use of receptor proteins and radiolabeled ligands, to measure the ability of test substances to bind to these receptors. The assay helps evaluate the potential endocrine-disrupting effects of chemicals by assessing their ability to interact with estrogen or androgen receptors, providing valuable information for regulatory and safety assessments.